食品科学 ›› 2009, Vol. 30 ›› Issue (18 ): 44-49.doi: 10.7506/spkx1002-6300-200918005

• 工艺技术 • 上一篇    下一篇

响应面法优化超声波辅助蛋白酶水解合浦珠母贝肉的条件研究

尚军 1,2,李来好1,吴燕燕1,*, 陈胜军 1,黄卉 1   

  1. 1.中国水产科学研究院南海水产研究所 2.广东海洋大学食品科技学院
  • 收稿日期:2009-05-27 出版日期:2009-09-15 发布日期:2010-12-29
  • 通讯作者: 吴燕燕 E-mail:wyy1028@tom.com
  • 基金资助:

    海南省重点科技项目计划项目(0712)

Response Surface Methodology for the Optimization of Ultrasound-assisted Enzymolysis Conditions of Pinctada martensii Muscle

SHANG Jun1,2,LI Lai-hao1,WU Yan-yan1,*,CHEN Sheng-jun1,HUANG Hui1   

  1. 1. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China;
    2. College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China
  • Received:2009-05-27 Online:2009-09-15 Published:2010-12-29
  • Contact: WU Yan-yan E-mail:wyy1028@tom.com

摘要:

利用响应面法优化超声波辅助酶解合浦珠母贝肉的工艺条件。单因素试验研究超声波功率、温度、超声时间对水解度的影响,在此基础上,应用Box-Behnken 中心组合设计建立数学模型,以水解度(DH)作为响应值,进行三因素三水平的响应面分析(Minitab)。结果表明,超声波辅助酶解合浦珠母贝肉的最佳工艺条件为:超声波功率160W、处理温度50℃、处理17min 后,水解1h 后产物水解度为31%,平均肽链长度3.2,与预测值的相对误差为0.5%。

关键词: 响应面法, 超声波, 合浦珠母贝肉, 辅助酶解

Abstract:

Ultrasound pretreatment was applied to the subtilisin-catalyzed hydrolysis of Pinctada martensii muscle. The effects of four ultrasound treatment parameters such as working mode. Power, temperature and treatment time on degree of hydrolysis (DH) of Pinctada martensii muscle were investigated by single-factor method. Subsequently, response surface methodology (RSM) based on a three-level, three-variable Box-Behnken experimental design was employed to evaluate the interactive effects of three crucial variables including power, temperature and treatment time. A mathematical model for DH as a function of the three variables was established. The optimum ultrasound treatment parameters for the subtilisin-catalyzed hydrolysis of Pinctada martensii muscle were determined as follows: power 160 W and temperature 50 ℃ for an ultrasound treatment duration of 17 min. Under the conditions, the actual DH after 1 h hydrolysis was 31%, and the average peptide chain length was 3.2 bp. The relative error between the predicted and the actual values was only 0.5%.

Key words: response surface methodology, ultrasonic, Pinctada martensii muscle, ultrasonic-assisted enzymolysis

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